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Also called: Autoclave maintenance, Servis autoclave

Autoclave Maintenance: Daily, Weekly and Annual Checklist

Most autoclave failures start with water, seals or skipped tests. This checklist keeps a clinic or lab steriliser passing — and lasting.

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Maintenance schedule

WhenTaskWhy
DailyWipe the door seal and chamber; check the water reservoir level and qualityDebris on the seal causes leaks; poor water causes scale and wet packs
DailyRun the Bowie-Dick or helix test (vacuum units)Proves air removal works before patient loads
WeeklyClean trays and the chamber with the maker’s cleaner; drain and rinse the reservoirRemoves residue and mineral build-up
WeeklyBiological (spore) testDefinitive proof that the cycle kills spores
MonthlyCheck and clean filters (bacteria filter, water filter, dust filter)Blocked filters slow drying and vacuum
YearlyProfessional service: calibration, valves, seal replacement, safety checksKeeps temperature and pressure readings accurate

Water: the most common cause of trouble

  • Use distilled or deionised water of the quality the manufacturer specifies.
  • Never top up with tap or mineral water — calcium and chlorides scale valves and stain instruments.
  • Empty and rinse the reservoir regularly so it never grows algae or biofilm.
  • If your unit has a water-quality sensor, never bypass its warning.

Common faults and what they usually mean

SymptomLikely cause
Wet packs at the end of the cycleOverloading, poor water, blocked filter, failing vacuum pump
Vacuum or leak test failsDirty or worn door seal, valve fault
Bowie-Dick shows uneven colour changeAir leak or poor air removal — stop using the machine
Stained or spotted instrumentsWater quality, detergent residue, mixed metals
Cycle aborts on temperature or pressureSensor drift, heating element or valve fault — needs a technician

Keeping records

Keep cycle printouts or logs, daily test results, spore test results and service reports together. They are the first thing an infection-control audit or licensing inspection will ask for.

Frequently asked questions

How often should an autoclave be serviced?
Most manufacturers specify a professional service at least once a year, plus daily and weekly user maintenance.
How often should the door seal be replaced?
Follow the manufacturer’s interval — often yearly or after a set number of cycles — and sooner if the leak test fails or the seal is cracked.
Why are my autoclave packs wet?
Usually overloading, poor-quality water, a blocked filter or a weak vacuum pump. Wet packs should be reprocessed, not used.

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